(Plenary, Oral and Poster Presentations)
[Plenary Sessions] [Bacteriology] [Immunology] [Parasitology] [Diagnostic Testing] [Virology] [Fish Health Surveys]
[Global Emergency Response & Preparedness] [Husbandry & Nutrition] [Shrimp & Lobster Disease] [Antimicrobial Use]
[Wildlife, Ornamental & Aquarium Medicine] [Mycoses] [Treatments] [Toxicology & Environmental Stress] [Additional Posters]






Mini-Plenary Presentations and Panel Discussion


PL-1 American Fisheries Society-Fish Health Section: Trish Barbash  
PL-2 Asian Fisheries Society-Fish Health Section:Ikuo Hirono  
PL-3 European Association of Fish Pathologists: Sven Bergmann
PL-4 International Association for Aquatic Animal Medicine: Ruth Francis-Floyd
PL-5 Japanese Society for Fish Pathology: Mamoru Yoshimizu
PL-6 National Shellfisheries Association: Roxanna Smolowitz





PL-7 Viral Hemorrhagic Septicemia Virus: An Old Virus with New Tricks: Gael Kurath
PL-8 Status of Finfish Culture and Parasitic Diseases in China: Tingbao Yang
PL-9 Novel Discoveries into the Immune System of Teleost Fish, and their Impact into the Future Development of Mucosal Vaccines: Oriol Sunyer
PL-10 Infectious Salmon Anemia in the Chilean Salmon Farming Industry: Origins and Impacts: Alicia Gallardo Lagno
PL-11 Short and Long Term Implications for Ecosystem Health in the North Central Gulf of Mexico Following the Deepwater Horizon Oil Spill: George Crozier










Bacteriology I (Session 1)

Chairs: Diane Elliott (USA) and Tom Wiklund (Finland)

S1-1      Intrinsic Resistance of Edwardsiella ictaluri to Cationic Antimicrobial Peptides is Mediated by UDP-Glc Dehydrogenase, an Enzyme Required for O-polysaccharide Synthesis

J Santander* and R Curtiss III


S1-2      Evaluation of the Edwardsiella ictaluri Type IV Secretion System Role in Virulence

                        AA Graff, ML Rogge and RL Thune*


S1-3      The Edwardsiella ictaluri Type III and Type IV Secretion Systems are Co-regulated and are Both Required for Virulence in the Channel Catfish

                        ML Rogge* and RL Thune


S1-4      Evaluation of the Role of Ammonia and Urea Transporters in the Pathogenesis of Edwardsiella ictaluri

                        J Beekman and RL Thune


S1-5      High Total Ammonia Nitrogen Concentrations Increase Survival of Channel Catfish Experimentally Infected with Flavobacterium columnare

                        AJ Mitchell* and B Farmer


S1-6      Ultrastructure of Skin Lesions on Channel Catfish, Ictalurus punctatus, Exposed to Flavobacterium columnare-Insights on Skin Discoloration and Epidermal Damage

                        A McElwain*, CR Arias, O Olivares-Fuster and SA Bullard

Bacteriology II (Session 8)

Chairs: Charles Gieseker (USA) and Bryndis Bjornsdottir (Iceland)

S8-1      Virulence Properties of Moritella viscosa Extracellular Products

B Bjornsdottir*, T Gudmundsdottir and BK Gudmundsdottir


S8-2      Detection of Yersinia ruckeri in Juvenile Cultured Atlantic Cod (Gadus morhua) in a Marine Nursery Located in Atlantic Canada

NY OŐBrien, DB Groman*, J Giles and DS Whelan


S8-3      Characterization of Isolates of Group B Streptococci from Diseased Farmed and Wild Marine Fish from the U.S. Gulf Coast

                        E Soto, R-X Wang, JE Wiles, W Baumgartner and JP Hawke*


S8-4      A Wide Analysis of Fish Bacterial Pathogen Streptococcus parauberis: Approach to Complimentary Genomics and Proteomics

                        SW Nho*, SB Park, HB Jang, IS Cha, MA Ha, YR Kim, J Hikima, M Othani, T Aoki and T-S Jung


S8-5      Ecological and Epidemiological Aspects of Bacterial Kidney Disease in Marine Phase Chinook Salmon

LD Rhodes*, CA Rice, CM Greene, DJ Teel, M Trudel, SL Nance, T Zubkowski, E Smith and P Moran


S8-6      Possible Common Etiological Agent for Strawberry Disease and Red Mark Syndrome in Rainbow Trout

M Metselaar*, KD Thompson, RML Gratacap, MJL Kik, SE LaPatra, SJ Lloyd, DR Call, PD Smith and A Adams

Bacteriology III (Session 19)

Chairs: Cynthia Stine (USA) and Matt Metselaar (Scotland) 

S19-1    Detection and Quantification of Renibacterium salmoninarum in Fluid Samples by Solid Phase Cytometry

                        CL McKibben, DG Elliott* and JL Drocourt


S19-2    Aeromonas salmonicida subsp. achromogenes and the Effect of the Autoinducer Synthase AsaI on Bacterial Virulence

                        J Schwenteit, T Gudmundsdottir, B Bjornsdottir and BK Gudmundsdottir*


S19-3    Weissella sp. Outbreaks in Brazilian Rainbow Trout (Oncorhynchus mykiss) Farms: Aetiological Agent, Genotyping, and Transmission Route, and Antibiotic Resistance Profile

                        HCP Figueiredo*, CAG Leal, FAA Costa and GA Carvalho-Castro


S19-4    Analysis of the Serological Variability of Vibro tapetis, Causative Agent of BRD in Clams

                        S Balboa, A Bastardo, A Dove, JL Barja, AE Toranzo and JL Romalde*


S19-5    Mycobacterial Infections in Resident Chesapeake Bay Striped Bass Morone saxatilis

CB Stine*, AM Baya, L Hungerford, MA Matsche, L Pieper, K Rosemary, C Driscoll and AS Kane


S19-6    Genetic Diversity of Mycobacterium chelonae in Aquatic Environments as Determined by PFGE

                        S Sanchez*, C Still, J Lane, J Saliki, AC Camus, G Bossart, T Clauss, T Mullican and A Dove

Bacteriology Posters

P-1     Eleven Years of Fish Bacterial Culture Isolates and Antimicrobial Susceptibility Testing at the Ocean‡rio de Lisboa, Portugal

L Sousa, T Albuquerque, M BraganŤa, N Baylina and N Pereira*


P-2     Analysis of Antibiotic Resistant Genes in Aquacultured Bacteria

J-Y Wang*, Y-J Geng, R-X Wang and J Feng


P-3     Development of Antimicrobial Resistance in Bacteria Associated to Reared Chilean Scallop Larvae Under Florfenicol Treatment

CD Miranda*, R Rojas and L Hurtado


P-4     Microbiological Conditions of a Sole (Solea senegalensis) Production Facility in Portugal

JA Sousa*, MC Cunha, JF Marques and MF Ramos


P-5     Klamath Basin Restoration and Endangered Species: Molecular Profiling of Mucosal Bacterial Communities

DD Iwanowicz*, CA Ottinger, KR Echols, T Wood, SP VanderKooi, BH Rosen and SM Burdick


P-6     Isolation and Characterization of Pathogenic Aeromonas hydrophila in Fishes in Northeast China

TY Lu*, D Wang, SW Li, HB Liu and JS Yin


P-7     Complete DNA Sequence and Analysis of the Large Virulence Plasmid of Edwardsiella tarda

JE Yu*, J Park and HY Kang


P-8     Investigation of Correlation Between CAMP Regulator Protein with Edwardsiella tarda Pathogenesis

                        J Park*, JE Yu and HY Kang


P-9     A Real-time Polymerase Chain Reaction for Detection and Quantification of Flavobacterium columnare from Two Different Genomovars

GD Gibbs*, MJ Manuel, MJ Griffin and ML Lawrence


P-10   Sequence Analysis of the 16S-23S Intergenic Spacer Regions of Flavobacterium columnare

L Ford*, E Soto, B Scheffler, ML Lawrence and LA Hanson


P-11   Survival of Flavobacterium columnare Under Starvation Conditions

S LaFrentz, O Olivares-Fuster and CR Arias*


P-12   Hemolytic Activity of Smooth and Rough Phenotypes of Flavobacterium psychrophilum

E Hšgfors-Ršnholm and T Wiklund*


P-13   Effect of pH and Temperature on Piscirickettsia salmonis

PA Smith*, P Fierro, MA Rojas, J Larenas and M Galleguillos


P-14   Searching for Extracellular Products of Piscirickettsia salmonis

MA Rojas, M Galleguillos, S Diaz, J Larenas and PA Smith*


P-15   Characterization of Pseudoalteromonas spp. with Pathogenic Potential for Cultured Clam Larvae

AL DiŽguez*, S Balboa, A Labella, D Castro, JJ Borrego and JL Romalde


P-16   Measurement of Viable Renibacterium salmoninarum Counts by Modified KDM-2 with Culture-spent Medium

M Yoshimizu*, T Matsui, T Nishizawa, H Kasai and M Kohara


P-17   Pathogenicity Mechanisms and Environmental Stressors in Streptococcal Challenges Using Nile Tilapia (Orechromis niloticus)

M Diez-Padrisa* and M Crumlish


P-18   Study on Manufacture Technology of Original Vibrio Culture for Binary Inactivated Bacterin

JF Tao*, YT Lai, Y Ren, GH Kang, Y Zhang, CB Shi and S Wu


P-19   Yersinia ruckeri Challenge on Rainbow Trout Fed Different Diet Types

L Madsen* and I Dalsgaard


P-20   Multiple Independent Emergence of Biotype-2 Yersinia ruckerei in the United States and Europe

TJ Welch* and DW Verner-Jeffreys




Immunology I: Vaccines (Session 2)
Chairs: Gregory Wiens (USA) and Oriol Sunyer (SPAIN/USA)  

S2-1      Expression Profiles of Cobia (Rachycentron canadum) Spleen and Head Kidney Following the Vaccination Against Vibrio carchariae, Proteus vulgaris and Streptococcus sp.

                        J Feng*, Z Guo, Y Su, L Xu, J-Y Wang, Y Hou and L Mao


S2-2      Attenuated Francisella asiatica iglC Mutant Induces Protective Immunity to Francisellosis in Tilapia

                        E Soto*, JE Wiles, PH Elzer, KR Macaluso and JP Hawke


S2-3      An Experimental Vaccine Against Aeromonas hydrophila can Induce Protection in Rainbow Trout

                        SE LaPatra*, KP Plant, WD Shewmaker, RA Burkhart and AW Morton


S2-4      Confirmation of Safety and Efficacy for a Live Attenuated Flavobacterium psychrophila Vaccine

                        TJ Johnson*, N Parvez, DR Call, BJ LaFrentz, T Miura and KD Cain


S2-5      Development, Characterization and Early Evaluation of New Modified Live Vaccines Against Columnaris Disease

                        CR Arias* and O Olivares-Fuster


S2-6      Initial Evaluation of an Edwardsiella ictaluri Type III Secretion System Mutant as a Vaccine Candidate Against Enteric Septicemia of Catfish

                        ML Rogge* and RL Thune

Immunology II (Session 18)

Chairs: Lora Petrie-Hansen (USA) and Nicole Kirchhoff (Tasmania)   

S18-1    Construction and Screening of a Library of Attenuated Mutants in Edwardsiella ictaluri, Identification of Genes Involves in Bacterial Invasion and Potential as Vaccines

                        S Menanteau-Ledouble* and ML Lawrence


S18-2    Efficacy of a Parenteral and Oral DNA Vaccine in Fish for Aquatic Mycobacteriosis

                        S Smith*


S18-3    The Identification and Characterization of Interleukin-17 Family Genes and Receptors in Fish

                        H Korenaga*, K Tomoya, K Haruka, T Hiroaki and S Masahiro


S18-4    Pathogenic Recognition Proteins in Rainbow Trout Plasma (Oncorhynchus mykiss)

                        S Russell*, K Young, M Edwards, A Peterson, A Reid and JS Lumsden


S18-5    Antibody Response in Ranched Southern Bluefin Tuna, Thunnus maccoyii, Naturally Infected with the Blood Fluke, Cardicola forsteri

                        NT Kirchhoff*, MJ Leef, V Valdenegro, C Hayward and BF Nowak


S18-6    The Zebrafish Model-A Flow Cytometry-based Investigation of Mechanisms of Endocytosis

                        CM Hohn*, S-R Lee, LM Pinchuk and L Petrie-Hanson

Immunology III (Session 25)

Chairs: Scott LaPatra (USA) and Takashi Aoki (Japan)

S25-1    Recombination Activation Gene 1 (Rag1) Mutant Zebrafish for Immunology Research

                        L Petrie-Hanson, CM Hohn* and LA Hanson


S25-2    Adhesion Dynamics of Flavobacterium columnare to Channel Catfish (Ictalurus punctatus) and Zebrafish (Danio rerio) after Immersion Challenge

                        O Olivares-Fuster, SA Bullard, A McElwain and CR Arias*


S25-3    Effects of the Anesthesia Agents Benzocaine and Tricane Methanesulfonate (MS-222) on Immune Parameters in Cuban Tree Frogs (Osteopilus septentrionalis) Tadpoles

                        BF Sears*, PW Snyder and JR Rohr


S25-4    Antiviral Activities of an Intracellular Viral RNA Sensor, MDA5, in Japanese Flounder (Paralichthys olivaceus)

                        M Ohtani*, J Hikima, T-S Jung and T Aoki


S25-5    Lymphocyte Deficient Zebrafish Demonstrate Specific Protection Following Bacterial Re-exposure

                        CM Hohn and L Petrie-Hanson*


S25-6    Generation of a Monoclonal Antibody Specific for ORF68 of Koi Herpesvirus

                        T Aoki*, I Hirono, H Kondo, T Takano, S Unajak, T Taki, J Hikima and T-S Jung

Immunology Posters


Immunology: Shrimp


P-39   A Newly Identified Anti-lipopolysaccharide Factor (ALF2) Gene in Kurama Shrimp Marsupenaeus japonicus

S Okugawa*, R Sudhakaran, M Inada, T Mekata, J Nishi, M Yoshimine, T Yoshida, Y Suzuki, T Kono, M Sakai and T Itami


P-40   Characterisation of the Genes Homologous to WSSV Genome in the Genomic DNA of Kuruma Shrimp,  Marsupenaeus japonicus

A Shitara*, H Yamada, T Koyama, MBB Maningas, H Kondo, T Aoki and I Hirono


P-41   Imiquimod and PolyI:C can Increase the Protection Against White Spot Disease Virus in Kuruma Shrimp, Marsupenaeus japonicus

Y Matsumoto, T Kono, H Korenaga, H Kuse, H Takayama, T Itami and M Sakai*


P-42   The Expression Analysis of Antimicrobial Peptide Genes in Kuruma Shrimp, Treated with Subunit Vaccine Against White Spot Disease

H Korenaga*, T Kono, H Kuse, H Takayama, T Itami and M Sakai


P-43   Shrimp Lysozyme is Important to Regulate Gram-negative Bacteria in Haemolymph

A Kaizu*, H Kondo, T Aoki and I Hirono


P-44   Molecular Cloning and Expression Analyses of Astakine Gene, MjAstakine, in Kuruma Shrimp Marsupenaeus japonicus

M Inada*, S Okugawa, J Nishi, M Yoshimine, R Sudhakaran, T Kono, T Mekata, T Yoshida, M Sakai and T Itami


P-45   Molecular Cloning and Analysis of c-Jun N-Terminal Kinase (JNK) in Kuruma Shrimp Marsupenaeus japonicus

M Yoshimine*, S Okugawa, M Inada, J Nishi, T Yoshida, Y Suzuki, T Kono, M Sakai, T Imata and T Mekata



Immunology: Fish


P-46   Identification of Deiodinase Type 3 as an Inducible Gene after Treatments of Bacterial Pathogen Associated Molecular Patterns

Y Kawana*, Y Suzuki, H Kondo, T Aoki and I Hirono


P-47   Antibiotic Intervention: Timing and the Affect on the Generation of Edwardsiella ictaluri Specific Antibody

                        TE Greenway*, MJ Griffin, DJ Wise, T Byars and J Walker


P-48   Immune Responses of Channel Catfish Following Exposure to a Temperature Tolerant Strain of Edwardsiella ictaluri

                        TE Greenway*, MJ Griffin, T Byars, J Walker and DJ Wise


P-49   Longitudinal Analysis of Anti-Edwardsiella ictaluri Antibody Response in Channel Catfish Fingerlings in the Pond Environment

                        TE Greenway*, DJ Wise, MJ Griffin, T Byars and J Walker


P-50   Solid Phase ELISA for Quantification of Antigen-Specific Antibody Responses in Channel Catfish: Analysis of Antibody Dilution Curves Following Multiple Antigen Exposures, Acute and Chronic Infections

            TE Greenway*, DJ Wise, MJ Griffin, J Walker and T Byars


P-51   Studies on Toll-like Receptors in Japanese Flounder, Paralichthys olivaceus

SD Hwang, H Kondo, I Hirono and T Aoki*


P-52   Genes Associated with Specific Protection in Rag1-/- Mutant Zebrafish

A Krishnavajhala*, CM Hohn, LA Hanson and L Petrie-Hanson


P-53   Clonal Growth of Carp (Cyprinus carpio) CD4 Single-positive αβT Cells In Vitro

T Yamaguchi, F Katakura and T Moritomo*




Parasitology I: Myxozoan Workshop 1 (Session 3)
Chairs: Jerri Bartholomew (USA) and Astrid Holzer (Spain)   

S3-1      An Overview of Myxozoan Parasites Found in Smallmouth Bass (Micropterus dolomieu) from Three Tributary Rivers of the Chesapeake Bay, USA

                        HL Ellery*, VS Blazer, LR Iwanowicz and DD Iwanowicz


S3-2      Sphaeromyxa sp (Myxosporea: Sphaeromyxidae) a Parasite of Lined Seahorses, Hippocampus erectus, from the Gulf of Mexico

                        BF Sears*, PA Anderson and EC Greiner


S3-3      Henneguya zschokkei s.l. (Myxozoa) in Whitefish (Coregonus lavaretus) in SW Finland, and Possible Interaction Between Wild and Farmed Fish

                        H-P Fagerholm*, B Szostakowska and T Wiklund


S3-4      Application of a Real-time PCR Assay for the Detection of Henneguya ictaluri in Channel Catfish Ponds

                        MJ Griffin*, LM Pote, AC Camus, MJ Manuel, TE Greenway and DJ Wise


S3-5      Predicting Salmon Mortality from Ceratomyxa shasta by Measuring Parasite Densities in Water Samples

                        SL Hallett*, RA Ray, RA Holt, SD Atkinson and JL Bartholomew


S3-6      Evaluation of the Risk of Myxobolus cerebralis Introduction as a Result of Fish Passage

                        CM Zielinski, SL Hallett and JL Bartholomew*


S3-7      Myxobolus Cerebralis in Fishes in Reservoirs of Kamchatka

                        TV Gavruseva*



Parasitology II: Myxozoan Workshop 2 (Session 7)

Chairs: Matt Griffin (USA) and Simon Jones (Canada)

S7-1      Spatial, Temporal and Host Factors Structure the Ceratomyxa shasta (Myxozoa) Population in the Klamath River Basin

                        SD Atkinson* and JL Bartholomew


S7-2      Host-specific Virulence and Transmission of Ceratomyxa shasta Genotypes I and II

                        CN Hurst* and JL Bartholomew


S7-3      The Dynamics of Mixed Myxozoan Infections and Potential Mechanisms of Within-host Interaction

                        AS Holzer*, R Wootten and C Sommerville


S7-4      Histopathological Features of Infections by the Myxosporean Zschokkella hildae Auerbach, 1910 in the Kidney of Atlantic Cod (Gadus morhua Linnaeus, 1758)

                        B Gorgoglione* and C Sommerville


S7-5      Chinook Salmon Host Response to Ceratomyxa shasta (Myxozoa)

                        SJ Bjork*, Y Zhang, O Sunyer and JL Bartholomew



Parasitology III: Myxozoan Workshop 3 (Session 10)

Chairs: Sascha Hallett (USA) and Oswaldo Palenzuela (Spain)        

Parasitology IV: Fish 1 (Session 15)

Chairs: Deborah Iwanowicz (USA) and Stephen Atkinson (Australia/USA)     

S15-1    A New Microsporidian from Common Stingray Dasyatis pastinaca in the Mediterranean Sea

                        A Diamant*, M Goren, MB Yokeş, BS Galil, Y Klopman, D Huchon, A Szitenberg and S† Karhan


S15-2    Diversity of Microsporidia among Marine Parasitic Copepods (Caligidae) in the Northeast Pacific Ocean

                        SRM Jones*, G Prosperi-Porta and E Kim


S15-3    Effects of Essential Oils on the Viability of Caligus rogercresseyi Using a Novel In Vitro Assay Based on the Inhibition of Lice Frontal Filament Formation

                        J Troncoso* and J Gonz‡lez


S15-4    Conceptual Framework for the Use of Parasites of Fishes as Bioindicators of Acute and Chronic Environmental Perturbation after the 2010 Deepwater Horizon Oil Spill (DHOS) in the Gulf of Mexico

                        SA Bullard* and GW Benz


S15-5    Molecular Identification, Ultrastructure and Histopathological Studies of Microsporidian Parasite of Transparent and Albino Puntius tetrazona

                        O Chang*, K Li, F Wang, C Liu and S Wu



Parasitology V: Shellfish (Session 20)

Chairs: Alistair Dove (USA) and Ole Samuelsen (Norway) 

S20-1    The Frequency and Distribution of Genetic Strains of Hematodinium, a Parasitic Dinoflagellate of the Blue Crab (Callinectes sapidus) along the Delmarva Peninsula, VA

                        KM Pagenkopp*, JD Shields, J Xiao, TL Miller, HJ Small, AR Place and KS Reece


S20-2    Discovery and Characterization of New Perkinsus Species

                        KS Reece*, GP Scott, CF Dungan, PM da Silva, C Adams, EA MacIntyre and

EM Burreson


S20-3    Haplosporidiosis in Bivalve Molluscs: New Perspectives in Haplosporidium costale, Agent of SSO Disease in Crassostrea virginica

                        NA Stokes* and RB Carnegie


S20-4    Oyster Parasites Common to Lake Worth Lagoon, Florida

                        SE Laramore* and J Scarpa


S20-5    Potential Bivalve Reservoirs of Bonamia sp. in FloridaŐs Indian River Lagoon

                        SE Laramore* and AL Lave


S20-6    Evolutionary Ecology of Crassostrea virginica and its Parasites

                        RB Carnegie* and EM Burreson



Parasitology VI: Fish 2 (Session 22)

Chairs: Linda Pote (USA) and Tingbao Yang (China)     

S22-1    Health and Reproductive Consequences for Sciaenops ocellatus (Perciformes: Sciaenidae) Infected with the Ovarian Parasite Philometra floridensis (Nematode: Philometridae)          MD Bakenhaster*, S Lowerre-Barbierrie, Y Kiryu, S Walters and EJ Fajer-Avila


S22-2    Unknown Clinostomum sp. Associated with Pathology in Channel Catfish (Ictalurus punctatus) in Mississippi (USA)

                        CM Doffitt*, LH Khoo, LM Pote and DT King


S22-3    DNA Barcoding Platyhelminths that Parasitize Aquatic Animals

                        SA Locke*, JD McLaughlin and DJ Marcogliese


S22-4    Life Histories of the Trematodes Found in Double-crested Cormorant Populations in the Mississippi Delta and the Potential Impact of these Parasites on Commercial and Wild Fish Species Found in this Region

                        MM OŐHear*, LM Pote, DT King, CM Doffitt, C Panuska and LE Miranda


S22-5    Infection Dynamics of Ecto-and Endoparasites of Channel Catfish (Ictalurus punctatus), Blue Catfish (Ictalurus furcatus), and C x B Hybrid Catfish (Female Ictalurus punctatus x Male Ictalurus furnacatus) in Earthen Pond Aquaculture

TN Truong* and SA Bullard


S22-6    Observation and Description of Five New Species of Cestode Parasites in Fishes from Ogba River, Benin City, Edo State, Nigeria

                        EE Obano*

Parasitology Posters


P-54   Spatial, Temporal and Host Factors Structure the Ceratomyxa shasta (Myxozoa) Population in the Klamath River Basin

SD Atkinson* and JL Bartholomew


P-55   A Survey of Myxosporean Parasites of Freshwater Dish and Invertebrates from the Pacific Northwest of North America

SD Atkinson*


P-56   Attachment, Cell-Cell Signaling, and Colony Formation in Spironucleus salmonis

                        SL Poynton* and MR Saghari Fard


P-57   An Update of Fish Parasites of Romania

CD Cojocaru*


P-58   Ectoparasites of Ornamental Fish Imported into Turkey

H Yavuzcan Yildiz*, G Sahin, E Veske, SF Secer, O Uluturk, AM Olguner, Z Gulen and G Erodogan


P-59   Freshwater Fish-borne Parasitic Zoonoses in Italy

A Gustinelli, M Caffara*, M Prearo and ML Fioravanti


P-60   DNA Barcoding and Phylogenetics of Clinostomum spp. from Africa, Europe, North and South America

M Caffara*, SA Locke, A Gustinelli, D Florio and ML Fioravanti


P-61   Acute Toxicity of Copper Sulfate to Bolbophorus sp. Cercariae

DJ Wise, MJ Griffin* and TE Greenway


P-62   A Duplex Real-time Polymerase Chain Reaction Assay for Differentiation Between Bolbophorus damnificus and Bolbophorus Type II sp. Cercariae

MJ Griffin*, DJ Wise, MC Yost, CM Doffitt, LM Pote, TE Greenway and LH Khoo


P-63   Shellfish Parasites as Ecological Indicators for Uruguayan Coastal Management: Applications in Traceability and Quality Control Certification of Seafood

EA Delgado*, D Carnevia, A Perretta, G Fabiano, X Lagos, D de Alava and R Cetrulo


Diagnostic Testing
(Session 5)

Chairs: Ron Thune (USA) and Masahiro Sakai (Japan)    

S5-1      Development of a U.S. National Fish Health Laboratory Network

                        AE Goodwin*, JB Rolland and KR Snekvik


S5-2      ICHV-2 (the Other Catfish Herpesvirus): Detection by qPCR

                        AE Goodwin*, E Marecaux and LA Hanson


S5-3      Use of Diagnostic Assays to Screen Rainbow Trout (Oncorhynchus mykiss) Broodstock for Flavobacterium psychrophilum

                        A Long*, DR Call and KD Cain


S5-4      Development of Immunochromatography Assay Kit for Rana Virus Determination

                        YR Kim, A Bayley, KD Thompson, A Adams, T Aoki and T-S Jung*


S5-5      Development of Two Novel Quantitative Polymerase Chain Reaction (PCR) Assays to Measure Alphaherpes Viral Load in Bottlenose Dolphins (Tursiops truncatus)

                        HTD Maness*, S Venn-Watson, LL Archer, R Rivera, ED Jensen and HH Nollens

Diagnostic Testing Posters  

P-23   Development and Evaluation of Real-time Loop-mediated Isothermal Amplifications Assay for Detection of Disease Viruses in Penaeid Shrimp

T Itami*, T Mekata, R Sudhakaran, T Kono, T Yoshida, Y Suzuki, K Supamattaya, M Inada, S Okugawa, J Nishi, M Yoshimine and M Sakai


P-24   Real-time PCR Detection of Water-borne Pathogens from Commercial Catfish Ponds

MJ Griffin*, DJ Wise, MJ Manuel, LM Pote, AC Camus, CM Doffitt, MC Yost, TE Greenway and LH Khoo


P-25   Hepatoglobin as a Candidate Biomarker of Domoic Acid Toxicosis in California Sea Lions

JA Ferrante*, B Neely, E Favre, JM Arthur, FMD Gulland, E Andrews, J Soper and MG Janech



(Session 16)
Chairs: Kyle Garver (Canada) and Lingbing Zeng (China)      

S16-1    Isolation and Identification of a Novel Reovirus by Using a Newly Established Cell Line Derived from the Kidney of Channel Catfish, Ictalurus punctatus, Rafinnesque

                        L Zeng*, J Xu, X Li, X Luo, Y Xiao and Y Zhou


S16-2    Re-activation of Koi Herpesvirus (KHV) in Persistent Infected Fish Used for Virus Detection after Non-lethal Sampling

                        SM Bergmann*


S16-3    Detection of KHV in Healthy Non-Carp Carrier Fish in Polish Inland Waters by PCR

                        J Kempter, M Kielpinski, R Panicz* and J Sadowski


S16-4    Analysis of Attenuation in a Gene 50 Deleted Channel Catfish Virus (Ictalurid herpesvirus 1) Construct Suggests that more is Involved than Lack of Gene 50 Expression

                        LA Hanson*, N Vanderheijden, JA Martial, D Kunec and L Ford


S16-5    A Fatal Reovirus is Highly Prevalent in Blue Crabs, Callinectes sapidus, in Aquaculture and Soft Shell Crab Production Facilities

                        HA Bowers, L Carrion, GA Messick, O Zmora, A Hanif, R Jagus and EJ Schott*


S16-6    Metagenomic Studies Reveal Novel Viruses in Shrimp

                        TFF Ng*, S Alavandi and M Breitbart

VIROLOGY II: VHS (Session 23)

Chairs: Luke Iwanowicz (USA) and David Verner-Jeffreys (England)    

S23-1    Results of 2009 Surveillance Efforts for Viral Hemorrhagic Septicemia Virus in Lake Ontario and Lake Superior

ER Cornwell*, GE Eckerlin, RG Getchell, GH Groocock, TH Thompson, WN Batts, G Kurath, JR Winton, PR Bowser, MB Bain and JW Casey


S23-2    Generation of a Mutant Viral Hemorrhagic Septicemia Virus of the Great Lakes Strain which is Attenuated in Yellow Perch (Perca flavescens)

                        A Ammayappan, TM Thompson, G Kurath and VN Vakharia*


S23-3    Development of Tools to Forecast the Potential for Viral Hemorrhagic Septicemia Epizootics in Wild Fish Populations

                        PK Hershberger*, JL Gregg, MK Purcell, CA Grady, JC Woodson and JR Winton


S23-4    Experimental Susceptibility of Sea Lamprey and Tiger Muskellunge to VHSV IVb

                        RG Getchell*, GH Groocock, ER Cornwell, LL Coffee, GA Wooster and PR Bowser


S23-5    Detection of VHSV IVb Within the Gonads of Great Lakes Fish using In Situ Hybridization

                        LJ Al-Hussinee* and JS Lumsden


S23-6    Humoral Immune Response of Muskellunge, Esox masquinongy, to Viral Hemorrhagic Septicemia Virus

                        EV Millard* and M Faisal


S23-7    Viral Hemorrhagic Septicemia (VHS) Surveillance on Wisconsin Fish Farms

                        MJ Kebus*

VIROLOGY III: IHN/ENV (Session 24)                                                                  

Chairs: Terry Ng (USA) and Mamoru Yoshimizu (Japan)


S24-1    Biology and Genetic Characterization of the Fish and Amphibian Herpesviruses (Family: Alloherpesviridae)

TB Waltzek*, GO Kelley, ME Alfaro, T Kurobe, AJ Davison and RP Hedrick


S24-2    Genogroup-specificity of DNA Vaccines against Infectious Hematopoietic Necrosis Virus (IHNV) in Rainbow Trout (Oncorhynchus mykiss)

                        MMD Pe–aranda*, SE LaPatra and G Kurath


S24-3    Vector Potential of the Salmon Louse (Lepeoptheirus salmonis) in the Transmission of Infectious Hematopoietic Necrosis Virus (IHNV)

                        E Jakob*, DE Barker and KA Garver


S24-4    Estimation of Viral Parameters Associated with Waterborne Transmission of Infectious Hematopoietic Necrosis Virus (IHNV) in Atlantic Salmon

                        KA Garver*, AM Grant and J Richard


S24-5    Molecular Characterization and Infection Kinetics of Erythrocytic Necrosis Virus (ENV) in Pacific Herring

                        EJ Emmenegger*, JA Glenn, WN Batts, CA Grady, JL Gregg, SE Roon, JR Winton and PK Hershberger



P-74   Preliminary Phylogenetic Analysis of Cheloniidae Fibropapilloma-associated Herpesvirus Sequences from Marine Turtles, Principe Island, Gulf of Guinea, West Africa

A Duarte*, S Gil, NS Loureiro, P Faisca, N Pereira and L Tavares


P-75   Effect of Low Water Temperature on Viral Infection and Replication of White Spot Syndrome Virus (WSSV) in Kuruma Shrimp, Marsupenaeus japonicus

LT Dang*, A Shitara, A Kaizu, H Kondo, T Aoki and I Hirono


P-76   Sensitivity of Chinook Salmon (Oncorhynchus tschawytscha) to Infectious Hematopoietic Necrosis Virus (Russia, Kamchatka)

SL Rudakova* and EV Bochkova


P-77   Distribution of Spring Viremia of Carp Virus (SVCV) in Carp

I Kiryu*, K Yuasa, M Kawana, N Ohseko and M Sano


P-78   VHSV Research Products Influencing Management and Regulatory Decisions

RG Getchell*, GH Groocock, ER Cornwell, KM Hope, RN Casey, JW Casey and PR Bowser

(Session 4)     

Chairs: John Foott (USA) and Jesus Lopez Romalde (Spain)    


S4-1      National Wild Fish Health Survey: Selected Findings and Limitations

SL Mumford*


S4-2      Demonstration of New Web Interface for the National Wild Fish Health Survey

KK Peters* and JL Bradley


S4-3      Results of Fish Health Assessment of Glass Eels, Anguilla rostrata, from Canadian Maritime Rivers from 2006-2010

                        DB Groman*, RW Threader, D Wadowska, T Maynard and L Bimke


S4-4      Cutthroat Trout Virus Surveys in New Mexico and Arizona

                        TD Lewis*, EM Rodarte and DD Hampton


S4-5      Epidemics and Control Strategies for Diseases of Farmed Salmonids: A Parameter Study

ART Jonkers, KJ Sharkey, M Thrush, JF Turnbull and K Morgan*





Chair: Roy Yanong (USA)                                                                                                      


S6-1      Sinking or Swimming in the OIE Aquatic Animal Health Code

                        MD Remmenga* and LL Gustafson


S6-2      Perspective on Global Preparedness for Aquatic Animal Diseases

                        KH Hartman*


S6-3      Developing, Implementing, Auditing and Certifying Veterinary Biosecurity Programs

                        DA Scarfe*


S6-4      Emerging Disease Issues and Concerns in Shellfish

                        R Smolowitz*    

S6-5      Pet Industry Efforts to Control Chytridiomycosis in Amphibians

                        N St-Erne*


S6-6      Aquarium and Aquaculture Facility Emergency Preparedness and Recovery

                  BR Whitaker*



HUSBANDRY AND NUTRITION (Session 9)                                                   

Chairs: John Drennan (USA) and Sally Anderson (New Zealand)                                    


S9-1      Isotonic Egg-washing Reduces Bacteria and Viruses on Salmon Eggs in Aquaculture

                        M Kohara*, S Ogawa, H Kasai and M Yoshimizu


S9-2      Bioexclusion in Site-level Biosecurity Plans

                        GA Karreman*


S9-3      Effects of Common Aquaculture Chemicals Against Selected Fish Pathogenic Bacteria

                        S Smith*


S9-4      The Importance of Hygiene for Live Feed Management: Lessons Learned from New Zealand Groper (Polyprion oxygeneios) Juveniles

S Anderson*, I Salinas, LA Tubbs, JS Lumsden, B Diggles, V Webb, Y Gublin, S Pether, G Irvine and S Walker





Chairs: Kim Reece (USA) and Ikuo Hirono (Japan)                                                      


S11-1    Molecular Immunology on Kuruma Shrimp, Marsupenaeus japonicus

I Hirono*, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagutao, H Yamada, K Komiya, T Aoki and H Kondo


S11-2    Characterization of Nitric Oxide Synthase Gene, MjNOS, in Kuruma Shrimp, Marsupenaeus japonicus and its mRNA Variants

M Inada*, S Okugawa, J Nishi, M Yoshimine, R Sudhakaran, T Kono, T Yoshida, M Sakai and T Itami


S11-3    Molecular Cloning and Characterization of Dicer2 Gene in Kurama Shrimp, Marsupenaeus japonicus

J Nishi*, S Okugawa, M Inada, T Yoshida, Y Suzuki, T Kono, M Sakai, T Mekata and T Itami


S11-4    Characterization of Tumor Necrosis Factor Ligand Superfamily Gene in Kuruma Shrimp, Marsupenaeus japonicus

S Okugawa*, R Sudhakaran, M Inada, T Mekata, J Nishi, M Yoshimine, T Yoshida, Y Suzuki, T Kono, M Sakai and T Itami


S11-5    Reciprocal Effects Between the Lethal Spiny Lobster Virus PaV1 and the Florida Lobster Fishery

DC Behringer*, JA Moss, JD Shields and MJ Butler IV


S11-6    Population Effects of Bitter Crab Disease on Snow and Tanner Crabs from Alaska and Newfoundland

                        JF Morado*, MSM Siddeek, DR Mullowney, EG Dawe, CA Shavey, PC Jensen, VC Lowe and RJ Cawthorn





Chairs: Ron Miller (USA) and Jennifer Matysczak (USA)                                                  


S12-1    Current Veterinary Approaches to Judicious Antimicrobial Use

                        DA Scarfe*, D Palić and CI Walster


S12-2    Aquaculture: Its Contribution to the Aquatic Resistome?

                        DW Verner-Jeffreys* and C Baker-Austin


S12-3    Efforts by the U.S. Food and Drug AdministrationŐs Center for Veterinary Medicine to Address Antimicrobial Resistance in Aquaculture

                        J Matysczak*


S12-4    Resistant or Susceptible May Not Always be Your Answer

                        RA Miller*


S12-5    Development of Antimicrobial Susceptibility Testing Methods for Flavobacterium columnare and F. psychrophilum

                        CM Gieseker* and TD Mayer


S12-6    Analysis of Bacterial Antibiotic Resistant Genes in Aquaculture

                        J-Y Wang*, Y-J Geng, R-X Wang and J Feng




Chairs: Brent Whitaker (USA) and Arik Diamant (Israel)                                                  


S13-1    Apicomplexan Protozoan Parasites in the Brain of an Immature Indo-Pacific Green Sea Turtle

                        M Flint*, JC Patterson-Kane, CJ Limpus and PC Mills


S13-2    The Importance of Emerging Exotic Viral Pathogens of Ornamental Fish in Australia

                        AE Rimmer*, JA Becker, A Tweedie and RJ Whittington


S13-3    Correlations of Swimming Patterns with Vertebral Deformities in Captive Sandtiger Sharks, Carcharias taurus

                        EE Tate*, PA Anderson and IK Berzins


S13-4    Prospecting for Biomarkers of Health in Whale Sharks, Rhinocodon typus, Using Metabolomic Methods

A Dove*, J Leisen, M Zhou, K Lim-Hing, DH Webb, L Gelbaum, J Byrne, M Viant, F Fernandez and J Kubanek


S13-5    Megalocytiviruses of Ornamental Fish and Implications for Aquaculture Species

                        J Go*, M Lancaster, K Deece, O Dhungyel and RJ Whittington


S13-6    Correlations of Capture, Transport, and Nutrition with Vertebral Deformities in Captive Sandtiger Sharks (Carcharias taurus)

                        PA Anderson*, DR Huber, DE Noaker and IK Bezkins



MYCOSES (Session 14)                                                                      

Chairs: Vicki Blazer (USA) and Sven Bergmann (Germany)                                           


S14-1    Ulcerative Mycoses Caused by Aphaomyces invadans and Ochroconis sp. in Wild and Cultured Fish Species

                        JP Hawke*, W Baumgartner and E Soto


S14-2    Exophialiosis in Bonita (Sarda chiliensis) from a Public Aquarium

                        K Hirokawa*, M Murray, A Reed and ES Weber III


S14-3    Effects of Environmental Temperature on the Kinetics of Ichthyophoniasis in Juvenile Pacific Herring (Clupea pallasii)

                        JL Gregg*, JJ Vollenweider, CA Grady, RA Heintz and PK Hershberger


S14-4    Molecular Detection of Ichthyophonus in Walleye Pollock and the Search for Planktonic Reservoirs Using Quantitative PCR

VC Lowe*, CS Friedman and JF Morado


TREATMENTS (Session 21)               

Chairs: Andrew Mitchell (USA) and Lincoln Tubbs (New Zealand/Canada)                         


S21-1    Comparison of In-Vitro and In–Vivo Efficacy of Florfenicol for Treatment of Francisella asiatica Infection in Tilapia

                        E Soto*, RG Endris and JP Hawke


S21-2    The Administration of a Bronopol-based Chemical Treatment to Control Ichthyopthirius multifiliis (Ciliophora) Infections

                        SM Pic—n-Camacho*, JE Bron and AP Shinn


S21-3    Efficacy of Florfenicol, Copper Sulphate and Potassium Permanganate in Controlling a Natural Infection of Aeromonas hydrophila and Flavobacterium columnare in Sunshine Bass, Morone chrysops Female x Morone saxatilis Male

                        AM Darwish*


S21-4    Evaluation of Novel Therapeutics against Monogenean Parasites

                        LA Tubbs*, W Bin and M Hamann


S21-5    Using Vaccines in Aquaculture

H Mitchell*




Chairs: John Hawke (USA) and Lone Madsen (Denmark)                                          


S26-1    Histopathological Effects of 17 Beta-estrodiol on the Gonads of Larval Xenopus laevis

                        JC Wolf*, I Lutz, W Kloas, TA Springer, HO Krueger, LR Holden and AJ Hosmer


S26-2    Mortalities, Skin Lesions and Intersex in Smallmouth Bass: Possible Associations with Contaminants of Emerging Concern

                        VS Blazer*, LR Iwanowicz, DD Iwanowicz and HL Ellery


S26-3    Investigating Fish Kills in Young-of-the-Year Smallmouth Bass in the Susquehanna River Drainage

                        L Iwanowicz*, VS Blazer, C Starliper, HL Ellery and K Crawford


S26-4    Disappearing Golden Shiner Phenomenon-Is Hydrogen Sulfide the Culprit?

                        AM Kelly* and S Kumaran




P-21   Haemocytic Neoplasia in Cultured Mediterranean Mussels (Mytilus galloprovincialis) in Slovene Sea

M Gombac, R Sitar and V Jencic*


P-22   Hematopoietic Neoplasm in the Redfin Needlefish, Strongylura notata (Beloniformes: Belonidae) from Estuaries of Florida, USA

Y Kiryu*, MD Bakenhaster, J Lansberg, M Tyler-Jedlund, P Wilson and J Fournie





P-26   High School Classroom Modules to Discern Effects of Environmental Stress Using an Oyster Model

JK Stephens*, MJ Koroly and AS Kane


P-27   Educational Opportunities in Aquatic Animal Health at the University of Florida

I Larkin*, M Walsh, R Yanong, BD Petty and R Francis-Floyd





P-28   Development of a Challenge Model for Visceral Toxicosis of Catfish

K Chatla*, P Gaunt and LA Hanson


P-29   Amoebic Systemic Diseases in Cultured Sole, Solea senegalensis

M Constenla, F Padr—s and O Palenzuela*

P-30   Aquatic Pathogen Database for Tracking Emerging Fish Virus Pathogens in North America

EJ Emmenegger*, E Kentop, TM Thompson, J Ranson, S Pittam, A Ryan, D Keon, JA Carlino, R Troyer, KA Garver, RB Life and G Kurath





P-31   Decompression as a Negative Factor in Pot Fisheries of Crabs in the Okhotsk Sea

TV Ryazanova*


P-32   Environmental Mycobacteria in Florida

SC Yarnell*, JFX Wellehan, Y Qiu, J Blackburn, JG Morris, M Lauzardo, D Ashkin, JA Johnson and AS Kane


P-33   Liver Pathologies Associated with Aquatic Pollution in the Lagos Lagoon Complex, Nigeria

OM Olarinmoye*


P-34   No Observable Adverse Effects Levels (NOAELs) for Fish and Pigs Fed Melamine and Cyanuric Acid

CB Stine*, J Ward, CM Gieseker, ER Evans, TD Mayer, N Hasbrouck, E Tall, C Nochetto and R Reimschuessel


P-35   A Case Study of Fish Deformities in the St. Lucie Estuary System, Florida, USA

AS Kane, D Reese* and JA Browder


P-36   Fish Health in a Florida Estuarine System in Relation to Canal Discharges and Water Quality

                        JA Browder, CM Nelson *, M Kandrashoff and F Kandrashoff


P-37   Linking Individual to Population Level Responses in the Eastern Mosquitofish (Gambusia holbrooki): Are Populations in Floridian Watersheds Impacted by Endocrine Disruptors?

EK Anderson*, LC Smith and DS Barber


P-38   Goiter in Captive Elasmobranchs: A Review

R Francis-Floyd*, A Morris, H Hamlin, M Walsh and L Guillette





P-64   International Registry of Coral Pathology

SM McLaughlin*, M Billmyre, D Howard and SS Tyler


P-65   Treasures in Archived Histopathology Collections: Preserving the Past for Future Understanding

DB Horowitz, EC Peters, I Sunila and JC Wolf*


P-66   Histology of the Alabama Rainbow, Villosa nebulosa (Bivalvia: Unionidae) Towards a Histological Atlas for the Freshwater Mussels

A McElwain*, CR Arias and SA Bullard





P-67   Metabolite Profiling for Predicting the Vaccine-response Substances of Flounder

D Hwang, S Kim, MS Kim and JY Cho*


P-68   Vaccination with Live Avirulent Edwardsiella tarda Strains Against Edwardsiellosis in Japanese Flounder

T Takano*, T Matsuyama, N Oseko, T Sakai, T Kamaishi, C Nakayasu, M Sano and T Iida


P-69   Survivability of Koi Herpesvirus (KHV) and a New Approach for Disinfection of KHV in Fish Breeding Effluent Water Using Anti-KHV Bacteria

N Yoshida, H Kasai* and M Yoshimizu


P-70   Toxicity Assessment of New Antifungal Combined Essential Oils in Comparison with Malachite Green to Rainbow Trout (Oncorhynchus mykiss)

SS Mirzargar*, SM Mousavi, HA Ebrahimzadeh Mousavi, R Omidbaigi and A Bahonar


P-71   Antimicrobial Substances from the Seaweed Ecklonia cava Against Bacterial Fish Pathogen Streptococcus iniae

S Kim, I Hong, D Hwang and JY Cho*


P-72   In Vitro Tests Using Natural-based Products Against the Theront Stages of the Marine Protozoan Cryptocaryon irritans Brown, 1951

SM Pic—n-Camacho*, MR Ruiz de Iba–ez, AS Holzer, M Arizcun Arizcun and P Mu–oz


P-73   In Vitro Tests Using Quinine-Based and Peracetic Acid-Based Products to Eliminate the Theront Stages of the Marine Protozoan Cryptocaryon irritans Brown, 1951

SM Pic—n-Camacho*, MR Ruiz de Iba–ez, AS Holzer, M Arizcun Arizcun and P Mu–oz





P-79   Evergreen Group and Research Center for Healthy Brookstock and Juvenile Production in South China

L Sedong


P-80   The United States National Marine Mammal Stranding Network: Past, Present, and Future

J Whaley*, J Cordaro, K Jackson, J Litz, D Marronel, K Wilkinson and T Rowles




Chairs: Jeff Wolf (USA) and Stephen Smith (USA)   


No Abstracts in this session.