SCIENTIFIC
PROGRAM
INTERNATIONAL PRIORITIES AND PERSPECTIVES:
Mini-Plenary Presentations
and Panel Discussion
American Fisheries Society-Fish Health
Section: Trish Barbash
Asian Fisheries Society-Fish Health Section: Ikuo Hirono
European Association of Fish Pathologists: Sven
Bergmann
International Association for Aquatic Animal Medicine:
Ruth Francis-Floyd
Japanese Society for Fish Pathology: Mamoru Yoshimizu
National Shellfisheries Association: Roxanna Smolowitz
PLENARY SESSIONS:
Viral Hemorrhagic Septicemia Virus: An Old Virus with New Tricks: Gael Kurath
Status of Finfish Culture and Parasitic Diseases in China: Tingbao Yang
Novel Discoveries into the
Immune System of Teleost Fish, and their Impact into the Future Development of
Mucosal Vaccines: Oriol
Sunyer
Infectious Salmon Anemia in the Chilean Salmon Farming Industry: Origins and Impacts: Alicia Gallardo Lagno
Short and Long Term Implications for Ecosystem Health in the North Central Gulf of Mexico Following the Deepwater Horizon Oil Spill: George Crozier
Bacteriology I (Session 1) Chairs: Diane Elliott (USA) and Tom Wiklund (Finland)
J Santander* and R Curtiss III
S1-2 Evaluation of the Edwardsiella ictaluri Type IV Secretion System Role in Virulence
AA
Graff, ML Rogge and RL Thune*
S1-3 The Edwardsiella ictaluri Type III and
Type IV Secretion Systems are Co-regulated and are Both Required for Virulence
in the Channel Catfish
ML
Rogge* and RL Thune
S1-4 Evaluation
of the Role of Ammonia and Urea Transporters in the Pathogenesis of Edwardsiella ictaluri
J
Beekman and RL Thune*
S1-5 High
Total Ammonia Nitrogen Concentrations Increase Survival of Channel Catfish
Experimentally Infected with Flavobacterium
columnare
AJ Mitchell* and B Farmer
S1-6 Ultrastructure
of Skin Lesions on Channel Catfish, Ictalurus
punctatus, Exposed to Flavobacterium
columnare-Insights on Skin Discoloration and Epidermal Damage
A
McElwain*, CR Arias, O Olivares-Fuster and SA Bullard
Chairs:
Charles Gieseker (USA) and Bryndis Bjornsdottir (Iceland) S8-1 Virulence Properties of Moritella viscosa Extracellular Products
B Bjornsdottir*, T Gudmundsdottir and BK
Gudmundsdottir
S8-2 Detection
of Yersinia ruckeri in Juvenile
Cultured Atlantic Cod (Gadus morhua)
in a Marine Nursery Located in Atlantic Canada
NY OŐBrien, DB Groman*, J Giles and DS Whelan
S8-3 Characterization
of Isolates of Group B Streptococci from Diseased Farmed and Wild Marine Fish
from the U.S. Gulf Coast
E
Soto, R-X Wang, JE Wiles, W Baumgartner and JP Hawke*
S8-4 A
Wide Analysis of Fish Bacterial Pathogen Streptococcus
parauberis: Approach to Complimentary Genomics and Proteomics
SW
Nho*, SB Park, HB Jang, IS Cha, MA Ha, YR Kim, J Hikima, M Othani, T Aoki and
S8-5 Ecological
and Epidemiological Aspects of Bacterial Kidney Disease in Marine Phase Chinook
Salmon
LD
Rhodes*, CA Rice, CM Greene, DJ Teel, M Trudel, SL Nance, T Zubkowski,
S8-6 Possible Common Etiological
Agent for Strawberry Disease and Red Mark Syndrome in Rainbow Trout
M Metselaar*, KD Thompson, RML Gratacap, MJL Kik, SE LaPatra,
SJ Lloyd, DR Call,
Bacteriology III (Session 19) Chairs: Cynthia Stine (USA) and Matt Metselaar
(Scotland) S19-1 Detection
and Quantification of Renibacterium
salmoninarum in Fluid Samples by Solid Phase Cytometry
CL
McKibben, DG Elliott* and JL Drocourt
S19-2 Aeromonas salmonicida subsp. achromogenes and the Effect of the
Autoinducer Synthase AsaI on Bacterial Virulence
J
Schwenteit, T Gudmundsdottir, B Bjornsdottir and BK Gudmundsdottir*
S19-3 Weissella sp. Outbreaks in Brazilian
Rainbow Trout (Oncorhynchus mykiss)
Farms: Aetiological Agent, Genotyping, and Transmission Route, and Antibiotic
Resistance Profile
HCP
Figueiredo*, CAG Leal, FAA Costa and GA Carvalho-Castro
S19-4 Analysis of the Serological Variability of Vibro tapetis, Causative Agent of BRD in
Clams
S
Balboa, A Bastardo, A Dove, JL Barja, AE Toranzo and JL Romalde*
S19-5 Mycobacterial Infections in Resident Chesapeake
Bay Striped Bass Morone saxatilis
CB
Stine*, AM Baya, L Hungerford, MA Matsche, L Pieper, K Rosemary, C Driscoll and
AS Kane
S19-6 Genetic Diversity of Mycobacterium chelonae in Aquatic Environments as Determined by
PFGE
S
Sanchez*, C Still, J Lane, J Saliki, AC Camus, G Bossart, T Clauss, T Mullican
and
P-1 Eleven
Years of Fish Bacterial Culture Isolates and Antimicrobial Susceptibility
Testing at the Ocean‡rio de Lisboa, Portugal
L Sousa, T Albuquerque, M BraganŤa,
N Baylina and N Pereira*
P-2 Analysis of
Antibiotic Resistant Genes in Aquacultured Bacteria
J-Y Wang*, Y-J Geng, R-X Wang and J Feng
P-3 Development
of Antimicrobial Resistance in Bacteria Associated to Reared Chilean Scallop
Larvae Under Florfenicol Treatment
CD Miranda*, R Rojas and L
Hurtado
P-4 Microbiological
Conditions of a Sole (Solea senegalensis)
Production Facility in Portugal
JA Sousa*, MC Cunha, JF
Marques and MF Ramos
P-5 Klamath
Basin Restoration and Endangered Species: Molecular Profiling of Mucosal
Bacterial Communities
DD Iwanowicz*, CA Ottinger, KR Echols, T Wood, SP
VanderKooi, BH Rosen and
P-6 Isolation and
Characterization of Pathogenic Aeromonas
hydrophila in Fishes in Northeast China
TY Lu*, D Wang, SW Li, HB Liu and JS Yin
P-7 Complete DNA Sequence
and Analysis of the Large Virulence Plasmid of Edwardsiella tarda
JE Yu*, J Park and HY Kang
P-8 Investigation
of Correlation Between CAMP Regulator Protein with Edwardsiella tarda Pathogenesis
J
Park*, JE Yu and HY Kang
P-9 A
Real-time Polymerase Chain Reaction for Detection and Quantification of Flavobacterium columnare from Two
Different Genomovars
GD Gibbs*, MJ Manuel, MJ Griffin and ML Lawrence
P-10 Sequence Analysis of the 16S-23S
Intergenic Spacer Regions of Flavobacterium
columnare
L Ford*, E Soto, B Scheffler, ML Lawrence and LA
Hanson
P-11 Survival of Flavobacterium columnare Under Starvation Conditions
S LaFrentz, O
Olivares-Fuster and CR Arias*
P-12 Hemolytic Activity of Smooth and
Rough Phenotypes of Flavobacterium
psychrophilum
E Hšgfors-Ršnholm and T Wiklund*
P-13 Effect of pH and Temperature on Piscirickettsia salmonis
PA Smith*, P Fierro, MA Rojas, J Larenas and M
Galleguillos
P-14 Searching for Extracellular
Products of Piscirickettsia salmonis
MA Rojas, M Galleguillos, S Diaz, J Larenas and PA
Smith*
P-15 Characterization of Pseudoalteromonas spp. with Pathogenic
Potential for Cultured Clam Larvae
AL DiŽguez*, S Balboa, A
Labella, D Castro, JJ Borrego and JL Romalde
P-16 Measurement
of Viable Renibacterium salmoninarum Counts by Modified KDM-2 with Culture-spent Medium
M Yoshimizu*, T Matsui, T
Nishizawa, H Kasai and M Kohara
P-17 Pathogenicity
Mechanisms and Environmental Stressors in Streptococcal Challenges Using Nile
Tilapia (Orechromis niloticus)
M Diez-Padrisa* and M Crumlish
P-18 Study on Manufacture
Technology of Original Vibrio Culture
for Binary Inactivated Bacterin
JF Tao*, YT Lai, Y Ren, GH Kang, Y Zhang, CB Shi and S
Wu
P-19 Yersinia
ruckeri Challenge on Rainbow Trout Fed Different Diet Types
L Madsen* and I Dalsgaard
P-20 Multiple Independent Emergence of
Biotype-2 Yersinia ruckerei in the
United States and Europe
TJ Welch* and DW Verner-Jeffreys
Immunology I: Vaccines (Session 2) S2-1 Expression
Profiles of Cobia (Rachycentron canadum)
Spleen and Head Kidney Following the Vaccination Against Vibrio carchariae, Proteus
vulgaris and Streptococcus sp.
J
Feng*, Z Guo, Y Su, L Xu, J-Y Wang, Y Hou and L Mao
S2-2 Attenuated Francisella asiatica iglC Mutant Induces Protective Immunity
to Francisellosis in Tilapia
E
Soto*, JE Wiles, PH Elzer, KR Macaluso and JP Hawke
S2-3 An Experimental Vaccine Against Aeromonas hydrophila can Induce
Protection in Rainbow Trout
SE
LaPatra*, KP Plant, WD Shewmaker, RA Burkhart and AW Morton
S2-4 Confirmation of Safety and Efficacy
for a Live Attenuated Flavobacterium
psychrophila Vaccine
TJ
Johnson*, N Parvez, DR Call, BJ LaFrentz, T Miura and KD Cain
S2-5 Development,
Characterization and Early Evaluation of New Modified Live Vaccines Against
Columnaris Disease
CR
Arias* and O Olivares-Fuster
S2-6 Initial
Evaluation of an Edwardsiella ictaluri Type III Secretion System Mutant as a Vaccine Candidate Against Enteric
Septicemia of Catfish
ML
Rogge* and RL Thune
Immunology II (Session 18) Chairs: Lora Petrie-Hansen (USA) and Nicole Kirchhoff
(Tasmania) S18-1 Construction
and Screening of a Library of Attenuated Mutants in Edwardsiella ictaluri, Identification of Genes Involves in
Bacterial Invasion and Potential as Vaccines
S
Menanteau-Ledouble* and ML Lawrence
S18-2 Efficacy of a Parenteral and Oral DNA Vaccine in
Fish for Aquatic Mycobacteriosis
S
Smith*
S18-3 The Identification and Characterization of
Interleukin-17 Family Genes and Receptors in Fish
H
Korenaga*, K Tomoya, K Haruka, T Hiroaki and S Masahiro
S18-4 Pathogenic Recognition Proteins in Rainbow Trout
Plasma (Oncorhynchus mykiss)
S
Russell*, K Young, M Edwards, A Peterson, A Reid and JS Lumsden
S18-5 Antibody
Response in Ranched Southern Bluefin Tuna, Thunnus
maccoyii, Naturally Infected with the Blood Fluke, Cardicola forsteri
NT
Kirchhoff*, MJ Leef, V Valdenegro, C Hayward and BF Nowak
S18-6 The Zebrafish Model-A Flow Cytometry-based
Investigation of Mechanisms of Endocytosis
CM
Hohn*, S-R Lee, LM Pinchuk and L Petrie-Hanson
Immunology III (Session 25) Chairs: Scott LaPatra (USA) and Takashi Aoki (Japan) S25-1 Recombination Activation Gene 1 (Rag1) Mutant
Zebrafish for Immunology Research
L
Petrie-Hanson, CM Hohn* and LA Hanson
S25-2 Adhesion
Dynamics of Flavobacterium columnare to Channel Catfish (Ictalurus punctatus)
and Zebrafish (Danio rerio) after
Immersion Challenge
O
Olivares-Fuster, SA Bullard, A McElwain and CR Arias*
S25-3 Effects
of the Anesthesia Agents Benzocaine and Tricane Methanesulfonate (MS-222) on
Immune Parameters in Cuban Tree Frogs (Osteopilus
septentrionalis) Tadpoles
BF
Sears*, PW Snyder and JR Rohr
S25-4 Antiviral
Activities of an Intracellular Viral RNA Sensor, MDA5, in Japanese Flounder (Paralichthys olivaceus)
M
Ohtani*, J Hikima, T-S Jung and T Aoki
S25-5 Lymphocyte Deficient Zebrafish Demonstrate
Specific Protection Following Bacterial
CM
Hohn and L Petrie-Hanson*
S25-6 Generation of a Monoclonal Antibody Specific for
ORF68 of Koi Herpesvirus
T
Aoki*, I Hirono, H Kondo, T Takano, S Unajak, T Taki, J Hikima and T-S Jung
Immunology Posters
Immunology: Shrimp
P-39 A
Newly Identified Anti-lipopolysaccharide Factor (ALF2) Gene in Kurama Shrimp Marsupenaeus japonicus
S
Okugawa*, R Sudhakaran, M Inada, T Mekata, J Nishi, M Yoshimine, T Yoshida,
P-40 Characterisation
of the Genes Homologous to WSSV Genome in the Genomic DNA of Kuruma Shrimp, Marsupenaeus japonicus
A Shitara*, H Yamada, T
Koyama, MBB Maningas, H Kondo, T Aoki and I Hirono
P-41 Imiquimod
and PolyI:C can Increase the Protection Against White
Spot Disease Virus in Kuruma Shrimp, Marsupenaeus
japonicus
Y Matsumoto, T Kono, H Korenaga,
H Kuse, H Takayama, T Itami and M Sakai*
P-42 The
Expression Analysis of Antimicrobial Peptide Genes in Kuruma Shrimp, Treated
with Subunit Vaccine Against White Spot Disease
H Korenaga*, T Kono, H Kuse, H
Takayama, T Itami and M Sakai
P-43 Shrimp Lysozyme is Important to
Regulate Gram-negative Bacteria in Haemolymph
A Kaizu*, H Kondo, T Aoki and
I Hirono
P-44 Molecular
Cloning and Expression Analyses of Astakine Gene, MjAstakine, in Kuruma Shrimp Marsupenaeus
japonicus
M Inada*, S Okugawa, J Nishi, M Yoshimine, R
Sudhakaran, T Kono, T Mekata,
P-45 Molecular
Cloning and Analysis of c-Jun N-Terminal Kinase (JNK) in Kuruma Shrimp Marsupenaeus japonicus
M
Yoshimine*, S Okugawa, M Inada, J Nishi, T Yoshida, Y Suzuki, T Kono, M Sakai,
Immunology: Fish
P-46 Identification
of Deiodinase Type 3 as an Inducible Gene after Treatments of Bacterial
Pathogen Associated Molecular Patterns
Y Kawana*, Y Suzuki, H Kondo, T Aoki and I Hirono
P-47 Antibiotic
Intervention: Timing and the Affect on the Generation of Edwardsiella ictaluri Specific Antibody
TE
Greenway*, MJ Griffin, DJ Wise, T Byars and J Walker
P-48 Immune
Responses of Channel Catfish Following Exposure to a Temperature Tolerant
Strain of Edwardsiella ictaluri
TE Greenway*, MJ Griffin, T Byars, J Walker and DJ
Wise
P-49 Longitudinal
Analysis of Anti-Edwardsiella ictaluri Antibody Response in Channel Catfish Fingerlings in the Pond Environment
TE
Greenway*, DJ Wise, MJ Griffin, T Byars and J Walker
P-50 Solid Phase ELISA for Quantification of
Antigen-Specific Antibody Responses in Channel Catfish: Analysis of Antibody
Dilution Curves Following Multiple Antigen Exposures, Acute and Chronic
Infections
TE
Greenway*, DJ Wise, MJ Griffin, J Walker and T Byars
P-51 Studies on Toll-like Receptors in
Japanese Flounder, Paralichthys olivaceus
SD Hwang, H Kondo, I Hirono and T Aoki*
P-52 Genes Associated with Specific
Protection in Rag1-/- Mutant Zebrafish
A Krishnavajhala*, CM Hohn, LA Hanson and L
Petrie-Hanson
P-53 Clonal Growth of Carp (Cyprinus
carpio) CD4 Single-positive αβT Cells In Vitro
T
Yamaguchi, F Katakura and T Moritomo*
Parasitology I: Myxozoan Workshop 1 (Session 3) Chairs: Jerri Bartholomew (USA) and Astrid Holzer (Spain) S3-1 An
Overview of Myxozoan Parasites Found in Smallmouth Bass (Micropterus dolomieu) from Three Tributary Rivers of the Chesapeake
Bay, USA
HL
Ellery*, VS Blazer, LR Iwanowicz and DD Iwanowicz
S3-2 Sphaeromyxa sp (Myxosporea:
Sphaeromyxidae) a Parasite of Lined Seahorses, Hippocampus erectus, from the Gulf of Mexico
BF
Sears*, PA Anderson and EC Greiner
S3-3 Henneguya zschokkei s.l. (Myxozoa) in
Whitefish (Coregonus lavaretus) in SW
Finland, and Possible Interaction Between Wild and Farmed Fish
H-P
Fagerholm*, B Szostakowska and T Wiklund
S3-4 Application
of a Real-time PCR Assay for the Detection of Henneguya ictaluri in Channel Catfish Ponds
MJ
Griffin*, LM Pote, AC Camus, MJ Manuel, TE Greenway and DJ Wise
S3-5 Predicting
Salmon Mortality from Ceratomyxa shasta by Measuring Parasite Densities in Water Samples
SL
Hallett*, RA Ray, RA Holt, SD Atkinson and JL Bartholomew
S3-6 Evaluation of the Risk of Myxobolus cerebralis Introduction as a
Result of Fish Passage
CM
Zielinski, SL Hallett and JL Bartholomew*
S3-7 Myxobolus Cerebralis in Fishes in
Reservoirs of Kamchatka
TV
Gavruseva*
Parasitology II: Myxozoan Workshop 2 (Session 7) Chairs: Matt Griffin (USA) and Simon Jones (Canada) S7-1 Spatial,
Temporal and Host Factors Structure the Ceratomyxa
shasta (Myxozoa) Population in the Klamath River Basin
SD
Atkinson* and JL Bartholomew
S7-2 Host-specific Virulence and Transmission
of Ceratomyxa shasta Genotypes I and
II
CN
Hurst* and JL Bartholomew
S7-3 The
Dynamics of Mixed Myxozoan Infections and Potential Mechanisms of Within-host
Interaction
AS
Holzer*, R Wootten and C Sommerville
S7-4 Histopathological
Features of Infections by the Myxosporean Zschokkella
hildae Auerbach, 1910 in the Kidney of Atlantic Cod (Gadus morhua Linnaeus, 1758)
B
Gorgoglione* and C Sommerville
S7-5 Chinook Salmon Host Response to Ceratomyxa shasta (Myxozoa)
SJ
Bjork*, Y Zhang, O Sunyer and JL Bartholomew
Parasitology III: Myxozoan Workshop 3 (Session 10) Chairs: Sascha Hallett (USA) and Oswaldo Palenzuela (Spain)
Chairs: Deborah Iwanowicz (USA) and Stephen Atkinson
(Australia/USA) S15-1 A New Microsporidian from Common Stingray Dasyatis pastinaca in the Mediterranean
Sea
A
Diamant*, M Goren, MB Yokeş, BS Galil, Y Klopman, D Huchon, A Szitenberg
and
S15-2 Diversity
of Microsporidia among Marine Parasitic Copepods (Caligidae) in the Northeast
Pacific Ocean
SRM
Jones*, G Prosperi-Porta and E Kim
S15-3 Effects
of Essential Oils on the Viability of Caligus
rogercresseyi Using a Novel In Vitro Assay Based on the Inhibition of Lice
Frontal Filament Formation
J
Troncoso* and J Gonz‡lez
S15-4 Conceptual
Framework for the Use of Parasites of Fishes as Bioindicators of Acute and
Chronic Environmental Perturbation after the 2010 Deepwater Horizon Oil Spill
(DHOS) in the Gulf of Mexico
SA
Bullard* and GW Benz
S15-5 Molecular
Identification, Ultrastructure and Histopathological Studies of Microsporidian
Parasite of Transparent and Albino Puntius
tetrazona
O Chang*, K Li, F Wang, C Liu and S Wu
Chairs: Alistair Dove (USA) and Ole Samuelsen (Norway) S20-1 The
Frequency and Distribution of Genetic Strains of Hematodinium, a Parasitic Dinoflagellate of the Blue Crab (Callinectes sapidus) along the Delmarva
Peninsula, VA
KM
Pagenkopp*, JD Shields, J Xiao, TL Miller, HJ Small, AR Place and KS Reece
S20-2 Discovery and Characterization of New Perkinsus Species
KS
Reece*, GP Scott, CF Dungan, PM da Silva, C Adams, EA MacIntyre and
EM Burreson
S20-3 Haplosporidiosis
in Bivalve Molluscs: New Perspectives in Haplosporidium
costale, Agent of SSO Disease in Crassostrea
virginica
NA
Stokes* and RB Carnegie
S20-4 Oyster Parasites Common to Lake Worth Lagoon,
Florida
SE
Laramore* and J Scarpa
S20-5 Potential Bivalve Reservoirs of Bonamia sp. in FloridaŐs Indian River
Lagoon
SE
Laramore* and AL Lave
S20-6 Evolutionary Ecology of Crassostrea virginica and its Parasites
RB
Carnegie* and EM Burreson
Parasitology VI: Fish 2 (Session 22) Chairs: Linda Pote (USA) and Tingbao Yang (China) S22-1 Health
and Reproductive Consequences for Sciaenops
ocellatus (Perciformes: Sciaenidae) Infected with the Ovarian Parasite Philometra floridensis (Nematode:
Philometridae)
MD
Bakenhaster*, S Lowerre-Barbierrie, Y Kiryu, S Walters and EJ Fajer-Avila
S22-2 Unknown Clinostomum sp. Associated with Pathology
in Channel Catfish (Ictalurus punctatus)
in Mississippi (USA)
CM
Doffitt*, LH Khoo, LM Pote and DT King
S22-3 DNA Barcoding Platyhelminths that Parasitize
Aquatic Animals
SA
Locke*, JD McLaughlin and DJ Marcogliese
S22-4 Life
Histories of the Trematodes Found in Double-crested Cormorant Populations in
the Mississippi Delta and the Potential Impact of these Parasites on Commercial
and Wild Fish Species Found in this Region
MM
OŐHear*, LM Pote, DT King, CM Doffitt, C Panuska and LE Miranda
S22-5 Infection
Dynamics of Ecto-and Endoparasites of Channel Catfish (Ictalurus punctatus), Blue Catfish (Ictalurus furcatus), and C x B Hybrid Catfish (Female Ictalurus punctatus x Male Ictalurus furnacatus) in Earthen Pond
Aquaculture
TN Truong* and SA Bullard
S22-6 Observation
and Description of Five New Species of Cestode Parasites in Fishes from Ogba
River, Benin City, Edo State, Nigeria
EE
Obano*
P-54 Spatial,
Temporal and Host Factors Structure the Ceratomyxa
shasta (Myxozoa) Population in the Klamath River Basin
SD Atkinson* and JL Bartholomew
P-55 A
Survey of Myxosporean Parasites of Freshwater Dish and Invertebrates from the
Pacific Northwest of North America
SD Atkinson*
P-56 Attachment, Cell-Cell Signaling,
and Colony Formation in Spironucleus
salmonis
SL
Poynton* and MR Saghari Fard
P-57 An Update of Fish Parasites of
Romania
CD Cojocaru*
P-58 Ectoparasites of Ornamental Fish
Imported into Turkey
H Yavuzcan Yildiz*, G Sahin, E Veske, SF Secer, O
Uluturk, AM Olguner, Z Gulen and
P-59 Freshwater Fish-borne Parasitic
Zoonoses in Italy
A Gustinelli, M Caffara*, M Prearo and ML Fioravanti
P-60 DNA
Barcoding and Phylogenetics of Clinostomum spp. from Africa, Europe, North and South America
M Caffara*, SA Locke, A Gustinelli, D Florio and ML
Fioravanti
P-61 Acute Toxicity of Copper Sulfate to Bolbophorus sp. Cercariae
DJ Wise, MJ Griffin* and TE Greenway
P-62 A Duplex Real-time Polymerase Chain
Reaction Assay for Differentiation Between Bolbophorus
MJ Griffin*, DJ Wise, MC Yost, CM Doffitt, LM Pote, TE
Greenway and LH Khoo
P-63 Shellfish
Parasites as Ecological Indicators for Uruguayan Coastal Management:
Applications in Traceability and Quality Control Certification of Seafood
EA Delgado*, D Carnevia, A
Perretta, G Fabiano, X Lagos, D de Alava and R Cetrulo
DIAGNOSTIC TESTING Diagnostic Testing
S5-1 Development of a U.S. National Fish
Health Laboratory Network
AE
Goodwin*, JB Rolland and KR Snekvik
S5-2 ICHV-2 (the Other Catfish
Herpesvirus): Detection by qPCR
AE
Goodwin*, E Marecaux and LA Hanson
S5-3 Use
of Diagnostic Assays to Screen Rainbow Trout (Oncorhynchus mykiss) Broodstock for Flavobacterium psychrophilum
A Long*, DR Call and KD Cain
S5-4 Development of Immunochromatography
Assay Kit for Rana Virus Determination
YR
Kim, A Bayley, KD Thompson, A Adams, T Aoki and T-S Jung*
S5-5 Development
of Two Novel Quantitative Polymerase Chain Reaction (PCR) Assays to Measure
Alphaherpes Viral Load in Bottlenose Dolphins (Tursiops truncatus)
HTD
Maness*, S Venn-Watson, LL Archer, R Rivera, ED Jensen and HH Nollens
P-23 Development and Evaluation of Real-time
Loop-mediated Isothermal Amplifications Assay for Detection of Disease Viruses
in Penaeid Shrimp
T Itami*, T Mekata, R Sudhakaran, T Kono, T Yoshida, Y
Suzuki, K Supamattaya,
P-24 Real-time PCR Detection of
Water-borne Pathogens from Commercial Catfish Ponds
MJ Griffin*, DJ Wise, MJ Manuel, LM Pote, AC Camus, CM
Doffitt, MC Yost,
P-25 Hepatoglobin as a Candidate
Biomarker of Domoic Acid Toxicosis in California Sea Lions
JA Ferrante*, B Neely, E Favre, JM Arthur, FMD
Gulland, E Andrews, J Soper and
VIROLOGY S16-1 Isolation
and Identification of a Novel Reovirus by Using a Newly Established Cell Line
Derived from the Kidney of Channel Catfish, Ictalurus
punctatus, Rafinnesque.
L
Zeng*, J Xu, X Li, X Luo, Y Xiao and Y Zhou
S16-2 Re-activation
of Koi Herpesvirus (KHV) in Persistent Infected Fish Used for Virus Detection
after Non-lethal Sampling
SM
Bergmann*
S16-3 Detection of KHV in Healthy Non-Carp Carrier Fish
in Polish Inland Waters by PCR
J
Kempter, M Kielpinski, R Panicz* and J Sadowski
S16-4 Analysis
of Attenuation in a Gene 50 Deleted Channel Catfish Virus (Ictalurid herpesvirus 1) Construct Suggests that more is Involved
than Lack of Gene 50 Expression
LA
Hanson*, N Vanderheijden, JA Martial, D Kunec and L Ford
S16-5 A
Fatal Reovirus is Highly Prevalent in Blue Crabs, Callinectes sapidus, in Aquaculture and Soft Shell Crab Production
Facilities
HA
Bowers, L Carrion, GA Messick, O Zmora, A Hanif, R Jagus and EJ Schott*
S16-6 Metagenomic Studies Reveal Novel Viruses in Shrimp
TFF
Ng*, S Alavandi and M Breitbart
VIROLOGY
II: VHS
Chairs: Luke Iwanowicz (USA) and David Verner-Jeffreys (England)
S23-1 Results
of 2009 Surveillance Efforts for Viral Hemorrhagic Septicemia Virus in Lake
Ontario and Lake Superior
ER
Cornwell*, GE Eckerlin, RG Getchell, GH Groocock, TH Thompson, WN Batts,
S23-2 Generation
of a Mutant Viral Hemorrhagic Septicemia Virus of the Great Lakes Strain which
is Attenuated in Yellow Perch (Perca
flavescens)
A
Ammayappan, TM Thompson, G Kurath and VN Vakharia*
S23-3 Development
of Tools to Forecast the Potential for Viral Hemorrhagic Septicemia Epizootics
in Wild Fish Populations
PK
Hershberger*, JL Gregg, MK Purcell, CA Grady, JC Woodson and JR Winton
S23-4 Experimental Susceptibility of Sea Lamprey and
Tiger Muskellunge to VHSV IVb
RG
Getchell*, GH Groocock, ER Cornwell, LL Coffee, GA Wooster and PR Bowser
S23-5 Detection of VHSV IVb Within the Gonads of Great
Lakes Fish using In Situ Hybridization
LJ
Al-Hussinee* and JS Lumsden
S23-6 Humoral
Immune Response of Muskellunge, Esox
masquinongy, to Viral Hemorrhagic Septicemia Virus
EV
Millard* and M Faisal
S23-7 Viral Hemorrhagic Septicemia (VHS) Surveillance on
Wisconsin Fish Farms
MJ
Kebus*
VIROLOGY III: IHN/ENV
Chairs: Terry Ng (USA) and Mamoru Yoshimizu (Japan)
S24-1 Biology
and Genetic Characterization of the Fish and Amphibian Herpesviruses (Family: Alloherpesviridae)
TB Waltzek*, GO Kelley, ME Alfaro, T Kurobe, AJ Davison and RP Hedrick
S24-2 Genogroup-specificity
of DNA Vaccines against Infectious Hematopoietic Necrosis Virus (IHNV) in
Rainbow Trout (Oncorhynchus mykiss)
MMD
Pe–aranda*, SE LaPatra and G Kurath
S24-3 Vector
Potential of the Salmon Louse (Lepeoptheirus
salmonis) in the Transmission of Infectious Hematopoietic Necrosis Virus
(IHNV)
E
Jakob*, DE Barker and KA Garver
S24-4 Estimation
of Viral Parameters Associated with Waterborne Transmission of Infectious
Hematopoietic Necrosis Virus (IHNV) in Atlantic Salmon
KA
Garver*, AM Grant and J Richard
EJ
Emmenegger*, JA Glenn, WN Batts, CA Grady, JL Gregg, SE Roon, JR Winton and
VIROLOGY POSTERS
P-74 Preliminary
Phylogenetic Analysis of Cheloniidae Fibropapilloma-associated Herpesvirus
Sequences from Marine Turtles, Principe Island, Gulf of Guinea, West Africa
A Duarte*, S Gil, NS Loureiro,
P Faisca, N Pereira and L Tavares
P-75 Effect
of Low Water Temperature on Viral Infection and Replication of White Spot
Syndrome Virus (WSSV) in Kuruma Shrimp, Marsupenaeus
japonicus
LT Dang*, A Shitara, A Kaizu, H Kondo, T Aoki and I
Hirono
P-76 Sensitivity
of Chinook Salmon (Oncorhynchus
tschawytscha) to Infectious Hematopoietic Necrosis Virus (Russia,
Kamchatka)
SL Rudakova* and EV Bochkova
P-77 Distribution of Spring Viremia of
Carp Virus (SVCV) in Carp
I Kiryu*, K Yuasa, M Kawana, N Ohseko and M Sano
P-78 VHSV Research Products Influencing
Management and Regulatory Decisions
RG Getchell*, GH Groocock, ER Cornwell, KM Hope, RN
Casey, JW Casey and
Chairs: John Foott (USA) and Jesus Lopez Romalde (Spain)
S4-1 National Wild Fish Health Survey:
Selected Findings and Limitations
SL Mumford*
S4-2 Demonstration of New Web Interface for
the National Wild Fish Health Survey
KK Peters* and JL Bradley
S4-3 Results
of Fish Health Assessment of Glass Eels, Anguilla
rostrata, from Canadian Maritime Rivers from 2006-2010
DB
Groman*, RW Threader, D Wadowska, T Maynard and L Bimke
S4-4 Cutthroat Trout Virus Surveys in New
Mexico and Arizona
TD
Lewis*, EM Rodarte and DD Hampton
S4-5 Epidemics and Control
Strategies for Diseases of Farmed Salmonids: A Parameter Study
ART Jonkers, KJ Sharkey, M
Thrush, JF Turnbull and K Morgan*
GLOBAL EMERGENCY RESPONSE AND PREPAREDNESS: EMERGING AQUATIC ISSUES
SPECIAL
SESSION BY THE AMERICAN VETERINARY MEDICAL ASSOCIATION
Chair: Roy Yanong (USA)
S6-1 Sinking or Swimming in the OIE Aquatic
Animal Health Code
MD
Remmenga* and LL Gustafson
S6-2 Perspective on Global Preparedness for
Aquatic Animal Diseases
KH
Hartman*
S6-3 Developing, Implementing, Auditing and
Certifying Veterinary Biosecurity Programs
DA
Scarfe*
S6-4 Emerging Disease Issues and Concerns
in Shellfish
R
Smolowitz* S6-5 Pet Industry Efforts to Control
Chytridiomycosis in Amphibians
N
St-Erne*
S6-6 Aquarium and Aquaculture Facility
Emergency Preparedness and Recovery
BR Whitaker*
HUSBANDRY AND NUTRITION
Chairs: John Drennan (USA) and Sally Anderson (New Zealand)
S9-1 Isotonic
Egg-washing Reduces Bacteria and Viruses on Salmon Eggs in Aquaculture
M
Kohara*, S Ogawa, H Kasai and M Yoshimizu
S9-2 Bioexclusion in Site-level Biosecurity
Plans
GA
Karreman*
S9-3 Effects of Common Aquaculture
Chemicals Against Selected Fish Pathogenic Bacteria
S
Smith*
S9-4 The
Importance of Hygiene for Live Feed Management: Lessons Learned from New
Zealand Groper (Polyprion oxygeneios)
Juveniles
S
Anderson*, I Salinas, LA Tubbs, JS Lumsden, B Diggles, V Webb, Y Gublin, S
Pether, G Irvine and S Walker
SHRIMP AND LOBSTER DISEASE AND IMMUNOLOGY
Chairs: Kim Reece (USA) and Ikuo Hirono (Japan)
S11-1 Molecular Immunology on Kuruma Shrimp, Marsupenaeus japonicus
I
Hirono*, T Koyama, A Kaizu, A Shitara, MBB Maningas, FF Fagutao, H Yamada,
S11-2 Characterization
of Nitric Oxide Synthase Gene, MjNOS, in Kuruma Shrimp, Marsupenaeus japonicus and its mRNA Variants
M Inada*, S Okugawa, J Nishi, M Yoshimine, R
Sudhakaran, T Kono, T Yoshida,
S11-3 Molecular
Cloning and Characterization of Dicer2 Gene in Kurama Shrimp, Marsupenaeus japonicus
J Nishi*, S Okugawa, M Inada, T Yoshida, Y Suzuki, T
Kono, M Sakai, T Mekata and
S11-4 Characterization
of Tumor Necrosis Factor Ligand Superfamily Gene in Kuruma Shrimp, Marsupenaeus japonicus
S Okugawa*, R Sudhakaran, M Inada, T Mekata, J Nishi,
M Yoshimine, T Yoshida,
S11-5 Reciprocal Effects Between the Lethal Spiny
Lobster Virus PaV1 and the Florida Lobster Fishery
DC Behringer*, JA Moss, JD Shields and MJ Butler IV
S11-6 Population
Effects of Bitter Crab Disease on Snow and Tanner Crabs from Alaska and
Newfoundland
JF
Morado*, MSM Siddeek, DR Mullowney, EG Dawe, CA Shavey, PC Jensen,
ANTIMICROBIAL USE IN AQUACULTURE: RESISTANCE, RESIDUES AND RESEARCH
SPECIAL
SESSION BY THE US FOOD AND DRUG ADMINISTRATION
Chairs: Ron Miller (USA) and Jennifer Matysczak (USA)
S12-1 Current Veterinary Approaches to Judicious
Antimicrobial Use
DA
Scarfe*, D Palić and CI Walster
S12-2 Aquaculture: Its Contribution to the Aquatic
Resistome?
DW
Verner-Jeffreys* and C Baker-Austin
S12-3 Efforts
by the U.S. Food and Drug AdministrationŐs Center for Veterinary Medicine to
Address Antimicrobial Resistance in Aquaculture
J
Matysczak*
S12-4 Resistant or Susceptible May Not Always be Your
Answer
RA
Miller*
S12-5 Development
of Antimicrobial Susceptibility Testing Methods for Flavobacterium columnare and F. psychrophilum
CM
Gieseker* and TD Mayer
S12-6 Analysis of Bacterial Antibiotic Resistant Genes in Aquaculture
J-Y
Wang*, Y-J Geng, R-X Wang and J Feng
WILDLIFE, ORNAMENTAL AND AQUARIUM MEDICINE
Chairs: Brent Whitaker (USA) and Arik Diamant (Israel)
S13-1 Apicomplexan Protozoan Parasites in the Brain of
an Immature Indo-Pacific Green Sea Turtle
M
Flint*, JC Patterson-Kane, CJ Limpus and PC Mills
S13-2 The Importance of Emerging Exotic Viral Pathogens
of Ornamental Fish in Australia
AE
Rimmer*, JA Becker, A Tweedie and RJ Whittington
S13-3 Correlations
of Swimming Patterns with Vertebral Deformities in Captive Sandtiger Sharks, Carcharias taurus
EE Tate*, PA Anderson and IK Berzins
S13-4 Prospecting
for Biomarkers of Health in Whale Sharks, Rhinocodon
typus, Using Metabolomic Methods
A Dove*, J Leisen, M Zhou, K Lim-Hing, DH Webb, L
Gelbaum, J Byrne, M Viant,
S13-5 Megalocytiviruses of Ornamental Fish and
Implications for Aquaculture Species
J
Go*, M Lancaster, K Deece, O Dhungyel and RJ Whittington
S13-6 Correlations
of Capture, Transport, and Nutrition with Vertebral Deformities in Captive
Sandtiger Sharks (Carcharias taurus)
PA
Anderson*, DR Huber, DE Noaker and IK Bezkins
Chairs: Vicki Blazer (USA) and Sven Bergmann (Germany)
S14-1 Ulcerative
Mycoses Caused by Aphaomyces invadans and Ochroconis sp. in Wild and
Cultured Fish Species
JP
Hawke*, W Baumgartner and E Soto
S14-2 Exophialiosis in Bonita (Sarda chiliensis) from a Public Aquarium
K
Hirokawa*, M Murray, A Reed and ES Weber III
S14-3 Effects
of Environmental Temperature on the Kinetics of Ichthyophoniasis in Juvenile
Pacific Herring (Clupea pallasii)
JL
Gregg*, JJ Vollenweider, CA Grady, RA Heintz and PK Hershberger
S14-4 Molecular
Detection of Ichthyophonus in Walleye
Pollock and the Search for Planktonic Reservoirs Using Quantitative PCR
VC Lowe*, CS Friedman and JF Morado
Chairs: Andrew Mitchell (USA) and Lincoln Tubbs (New Zealand/Canada)
S21-1 Comparison
of In-Vitro and In–Vivo Efficacy of Florfenicol for Treatment of Francisella asiatica Infection in Tilapia
E
Soto*, RG Endris and JP Hawke
S21-2 The
Administration of a Bronopol-based Chemical Treatment to Control Ichthyopthirius multifiliis (Ciliophora)
Infections
SM
Pic—n-Camacho*, JE Bron and AP Shinn
S21-3 Efficacy
of Florfenicol, Copper Sulphate and Potassium Permanganate in Controlling a
Natural Infection of Aeromonas hydrophila and Flavobacterium columnare in
Sunshine Bass, Morone chrysops Female x Morone saxatilis Male
AM
Darwish*
S21-4 Evaluation of Novel Therapeutics against
Monogenean Parasites
LA
Tubbs*, W Bin and M Hamann
S21-5 Using Vaccines in Aquaculture
H Mitchell*
TOXICOLOGY AND ENVIRONMENTAL STRESS
Chairs: John Hawke (USA) and Lone Madsen (Denmark)
S26-1 Histopathological Effects of 17 Beta-estrodiol on
the Gonads of Larval Xenopus laevis
JC
Wolf*, I Lutz, W Kloas, TA Springer, HO Krueger, LR Holden and AJ Hosmer
S26-2 Mortalities,
Skin Lesions and Intersex in Smallmouth Bass: Possible Associations with
Contaminants of Emerging Concern
VS
Blazer*, LR Iwanowicz, DD Iwanowicz and HL Ellery
S26-3 Investigating
Fish Kills in Young-of-the-Year Smallmouth Bass in the Susquehanna River
Drainage
L
Iwanowicz*, VS Blazer, C Starliper, HL Ellery and K Crawford
S26-4 Disappearing Golden Shiner Phenomenon-Is
Hydrogen Sulfide the Culprit?
AM
Kelly* and S Kumaran
P-21 Haemocytic
Neoplasia in Cultured Mediterranean Mussels (Mytilus galloprovincialis) in Slovene Sea
M Gombac, R Sitar and V
Jencic*
P-22 Hematopoietic
Neoplasm in the Redfin Needlefish, Strongylura
notata (Beloniformes: Belonidae) from Estuaries of Florida, USA
Y Kiryu*, MD Bakenhaster, J Lansberg, M Tyler-Jedlund,
P Wilson and J Fournie
EDUCATION POSTERS
P-26 High
School Classroom Modules to Discern Effects of Environmental Stress Using an
Oyster Model
JK Stephens*, MJ Koroly and AS Kane
P-27 Educational
Opportunities in Aquatic Animal Health at the University of Florida
I Larkin*, M Walsh, R
Yanong, BD Petty and R Francis-Floyd
EMERGING DISEASE POSTERS
P-28 Development of a Challenge Model
for Visceral Toxicosis of Catfish
K Chatla*, P Gaunt and LA Hanson
P-29 Amoebic Systemic Diseases in
Cultured Sole, Solea senegalensis
M Constenla, F Padr—s and O
Palenzuela* P-30 Aquatic
Pathogen Database for Tracking Emerging Fish Virus Pathogens in North America
EJ Emmenegger*, E Kentop, TM Thompson, J Ranson, S
Pittam, A Ryan, D Keon,
HEALTH, DISEASE AND THE ENVIRONMENT POSTERS
P-31 Decompression as a Negative Factor
in Pot Fisheries of Crabs in the Okhotsk Sea
TV Ryazanova*
P-32 Environmental Mycobacteria in
Florida
SC Yarnell*,
JFX Wellehan, Y Qiu, J Blackburn, JG Morris, M Lauzardo, D Ashkin,
P-33 Liver Pathologies Associated with
Aquatic Pollution in the Lagos Lagoon Complex, Nigeria
OM Olarinmoye*
P-34 No
Observable Adverse Effects Levels (NOAELs) for Fish and Pigs Fed Melamine and
Cyanuric Acid
CB Stine*, J Ward, CM Gieseker, ER Evans, TD Mayer,
N Hasbrouck, E Tall, C Nochetto and R
Reimschuessel
P-35 A Case Study of Fish Deformities in
the St. Lucie Estuary System, Florida, USA
AS Kane, D Reese* and JA Browder
P-36 Fish Health in a Florida Estuarine
System in Relation to Canal Discharges and Water Quality
JA Browder, CM Nelson *,
M Kandrashoff and F Kandrashoff
P-37 Linking
Individual to Population Level Responses in the Eastern Mosquitofish (Gambusia holbrooki): Are Populations in Floridian Watersheds Impacted by
Endocrine Disruptors?
EK Anderson*, LC Smith and DS Barber
P-38 Goiter in Captive Elasmobranchs: A Review
R Francis-Floyd*, A Morris, H Hamlin, M Walsh and L
Guillette
PATHOLOGY REFERENCE COLLECTION POSTERS
P-64 International Registry of Coral
Pathology
SM McLaughlin*, M Billmyre, D Howard and SS Tyler
P-65 Treasures in Archived
Histopathology Collections: Preserving the Past for Future Understanding
DB Horowitz, EC Peters, I Sunila and JC Wolf*
P-66 Histology
of the Alabama Rainbow, Villosa nebulosa (Bivalvia: Unionidae) Towards a Histological Atlas for the Freshwater Mussels
A McElwain*, CR Arias and SA Bullard
VACCINE AND TREATMENT POSTERS
P-67 Metabolite Profiling for Predicting
the Vaccine-response Substances of Flounder
D Hwang, S Kim, MS Kim and JY Cho*
P-68 Vaccination
with Live Avirulent Edwardsiella tarda Strains Against Edwardsiellosis in Japanese Flounder
T Takano*, T Matsuyama, N Oseko,
T Sakai, T Kamaishi, C Nakayasu, M Sano and
P-69 Survivability
of Koi Herpesvirus (KHV) and a New Approach for Disinfection of KHV in Fish Breeding
Effluent Water Using Anti-KHV Bacteria
N Yoshida, H Kasai* and M
Yoshimizu
P-70 Toxicity
Assessment of New Antifungal Combined Essential Oils in Comparison with
Malachite Green to Rainbow Trout (Oncorhynchus
mykiss)
SS Mirzargar*, SM Mousavi, HA Ebrahimzadeh Mousavi, R
Omidbaigi and A Bahonar
P-71 Antimicrobial
Substances from the Seaweed Ecklonia cava Against Bacterial Fish Pathogen Streptococcus
iniae
S Kim, I Hong, D Hwang and JY Cho*
P-72 In Vitro Tests Using
Natural-based Products Against the Theront Stages of the Marine Protozoan Cryptocaryon irritans Brown, 1951
SM Pic—n-Camacho*, MR Ruiz de Iba–ez, AS Holzer, M
Arizcun Arizcun and P Mu–oz
P-73 In Vitro Tests Using Quinine-Based and Peracetic
Acid-Based Products to Eliminate the Theront
Stages of the Marine Protozoan Cryptocaryon
irritans Brown, 1951
SM Pic—n-Camacho*, MR Ruiz de Iba–ez, AS Holzer, M
Arizcun Arizcun and P Mu–oz
PROGRAM ADDITION POSTERS
P-79 Evergreen Group and Research Center for
Healthy Brookstock and Juvenile Production in South China
L Sedong
P-80 The United States National Marine Mammal Stranding
Network: Past, Present, and Future
J Whaley*, J Cordaro, K Jackson, J
Litz, D Marronel, K Wilkinson and T Rowles
DIAGNOSTIC CHALLENGES
SESSION SUPPORTED BY THE
DEPARTMENT OF MOLECULAR AND COMPARATIVE PATHOBIOLOGY, JOHN HOPKINS UNIVERSITY
SCHOOL OF MEDICINE, BALTIMORE, USA
Chairs: Jeff Wolf (USA) and Stephen Smith (USA)
No Abstracts in this session.
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